Developing caryopses of barley (Hordeum vulgare L. cv.
Golf) were
monitored for starch-branching enzyme
activity for 1 month after anthesis. Homogenized caryopses from grains
with
the highest specific activity were used
as starting material for purification of branching enzyme by FPLC chromatography.
Several branching enzyme
activity fractions were resolved. We have previously described the isolation
of
a 51/50 kDa protein with starch
branching activity from one of these fractions. From three other fractions,
we
have now partially purified starch
branching enzymes corresponding to forms I, IIa and IIb.
