During their growth in vitro, promastigotes of Leishmania
amazonensis undergo differentiation from complement-susceptible to
complement-resistant forms. Here, we demonstrate that both forms bind
comparable amounts of C3 on their
surfaces, with the predominant molecule species being the haemolytically
active C3b. Likewise, equivalent amounts of C9
are deposited on both forms of promastigotes. However, while C9-bearing
complexes are exposed on the cell surface of
resistant promastigotes, they are cryptic in the susceptible stage of the
parasites. The membrane fraction of complement-resistant promastigote
lysates has the ability to inhibit complement-mediated haemolysis,
blocking C9, but not C3
deposition to complement-activating complexes. Moreover, the membrane
fraction of complement-resistant promastigote
lysates can inhibit the late steps of guinea-pig erythrocyte lysis much
more
efficiently than complement-susceptible ones.
Our results indicate that L. amazonensis promastigotes evade
complement killing by inhibiting the cytolytic pathway of
the complement cascade.