Many end-to-end, large vocabulary, continuous speech recognition systems are now able to achieve better speech recognition performance than conventional systems. Most of these approaches are based on bidirectional networks and sequence-to-sequence modeling however, so automatic speech recognition (ASR) systems using such techniques need to wait for an entire segment of voice input to be entered before they can begin processing the data, resulting in a lengthy time-lag, which can be a serious drawback in some applications. An obvious solution to this problem is to develop a speech recognition algorithm capable of processing streaming data. Therefore, in this paper we explore the possibility of a streaming, online, ASR system for Japanese using a model based on unidirectional LSTMs trained using connectionist temporal classification (CTC) criteria, with local attention. Such an approach has not been well investigated for use with Japanese, as most Japanese-language ASR systems employ bidirectional networks. The best result for our proposed system during experimental evaluation was a character error rate of 9.87%.

Glycosaminoglycans (GAGs) are present in the oviduct in which the major part of sperm capacitation occurs. In this study we have tested how capacitation of frozen-thawed bull spermatozoa is effected by exposure to different GAGs detectable or possibly present in oviductal fluid; i.e. heparin, hyaluronan, heparan sulphate, dermatan sulphate and chondroitin sulphate. Following exposure of different duration, the spermatozoa were stained with either Chlortetracycline (CTC) or merocyanine-540 and evaluated with epifluorescent light microscopy or flow cytometry, respectively. Heparin elicited a significant increase in the number of alive, capacitated spermatozoa, either expressed as higher merocyanine-540 fluorescence (p < 0.0001) or as B-pattern (p = 0.0021) in the CTC assay, during 4 h of incubation. When comparing the different GAG treatments one by one to the negative control in the flow cytometric study, only heparin and dermatan sulphate were significant (p < 0.0001) higher than the control at 0–30 min of incubation. Duration of incubation did not affect the proportion of capacitated spermatozoa when measured as merocyanine-540 fluorescence or CTC B-pattern, but the length of the incubation did affect the number of dead (Yo-PRO 1 positive) spermatozoa (p < 0.0001). Exposure to zona pellucida proteins significantly increased the proportion of acrosome reacted spermatozoa (p = 0.016). Both heparin and dermatan sulphate induce capacitation of frozen-thawed bull spermatozoa in vitro.