The assayers’ ingots recovered in 2014 from the SS Central America contain geochemical signatures and anomalies which convey information about the culture of miners and assayers in the late 1850s. A significant number of ingots (21%) contain elevated copper and zinc in the same ratio as a common brass alloy of the period, suggesting that adulteration of placer gold dust was a common issue. The ingots with the brass-like signature also have a gold fineness much lower than measured for typical California placer gold. Trace elements like palladium in uniform concentrations in all Kellogg & Humbert ingots suggest the use of quality high-temperature tools, while lead and tin in all ingots suggest specific contamination and industrial hygiene issues common to the whole industry. Other trace constituents such as arsenic, antimony and bismuth are likely to have originated from dense minerals associated with placer gold, or from the natural placer gold alloy itself. Comparison of modern analysis of ingots vs. assay values stamped on the ingots themselves suggests some firms like Kellogg & Humbert did superior assay work, while others like the San Francisco office of Justh & Hunter probably had issues with quality control. The variations in assay ingot alloy chemistry provide a window into this important period in American history.