This article explores the analytical trajectory of desecuritisation strategies in the Global South through the case of Colombian refugees in Ecuador (2005–12). It maps desecuritisation strategies and their enabling and constraining factors against the backdrop of an entrenched infiltration discourse and an emerging rights-based discourse. The analysis of speeches, interviews, and policies demonstrates that governmental elites set in motion more transformative strategies when regional identity and emigration are raised in the political agenda. However, critical developments such as bilateral tensions and the lack of audience support sway desecuritisation towards more managerial strategies and ultimately, to resecuritisation. Shifting the empirical application of desecuritisation to this South American setting reveals the transformational capacity of desecuritisation strategies and reiterates the decisive role of the audience.