Field observations in Ohio suggested a possible interaction between soybean cyst nematode (SCN) and glyphosate in Countrymark 316 soybean, a transgenic glyphosate-resistant (R) variety that also expresses SCN resistance derived from ‘PI88788’ soybean. To investigate this possible interaction under controlled conditions, greenhouse experiments were conducted in which Countrymark 316 (R) and Corsoy 79 (S = susceptible to glyphosate and SCN) soybean root and shoot weights were measured in response to three concentrations of race 3 SCN inoculum and five glyphosate doses. Experiments were conducted in which glyphosate was applied 9, 18, or 27 d after inoculation (DAI). Data from SCN treatments in the (S) soybean experiments were regressed on glyphosate dosage and fit to a log-logistic dose–response model. SCN–glyphosate interaction in the (S) soybean variety reduced root dry weight of SCN-inoculated plants 6% compared with noninoculated plants when 1.0 kg/ha glyphosate was applied 18 DAI. The most significant interaction of SCN and glyphosate in the (S) variety occurred when glyphosate was applied 27 DAI; the glyphosate dose required to reduce shoot fresh weight 25% was 0.55 kg/ha in the noninoculated control compared with 0.32 kg/ha in plants inoculated with 1,000 SCN eggs/200 cm3 soil. Glyphosate rates of 0.84 and 1.69 kg/ha reduced root dry weights of Countrymark 316 (R) soybean 10 to 13% at the 18 DAI application timing only. Inoculation with SCN reduced shoot fresh weight of (R) soybean 8 to 29% across all experiments, but there was no interaction of glyphosate and SCN in (R) soybean.

Based on field surveys and evaluations in the greenhouse, two fungal pathogens, Bipolaris sacchari and Drechslera gigantea, were identified as promising biological control agents for cogongrass. In greenhouse trials, the application of spore suspensions of these fungi containing 105 spores/ ml in a 1% aqueous gelatin solution to cogongrass plants and their incubation in a dew chamber for 24 h resulted in disease symptoms that ranged from discrete lesions to complete blighting of leaves. Disease severity (DS), based on a rating scale for southern corn leaf blight with 50% as the maximum DS rating, ranged from 42 to 49%. In greenhouse experiments, the application of spores formulated in an oil emulsion composed of 4% horticultural oil, 10% light mineral oil, and 86% water resulted in higher levels of foliar blight with no dew exposure or shorter periods of dew exposure (4, 8, or 12 h) as compared with the application of spores formulated in 1% gelatin. Field trials demonstrated that under natural conditions, the application of a spore and an oil emulsion mixture containing 105 spores/ml of either fungus could cause foliar injury from disease and phytotoxic damage from the oil emulsion. Depending on the application rate (100 or 200 ml/plot), the level of foliar injury ranged from 40 to 86% (based on a field assessment scale of 0 to 100% foliar injury) with B. sacchari as the test fungus. However, with D. gigantea as the test fungus, foliar injury ranged from 9 to 70% depending on the application volume and the oil concentration used. Although B. sacchari and D. gigantea were capable of causing foliar blight on cogongrass, the regenerative ability of the rhizomes allowed cogongrass to recover from the damage caused by these fungi. However, the level of injury caused by these fungi is sufficient to support their use as components for integrated management of cogongrass.