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3 results

  • A novel microfluidic device for human sperm separation based on rheotaxis

  • Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass
  • Journal:
    Zygote , FirstView
    Published online by Cambridge University Press:
    27 December 2024, pp. 1-9
    • Article
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  • This study explores the efficacy of a novel microfluidic device in isolating rheotactic sperm and assesses their advantages compared with other motile sperm. Two microfluidic devices were used in this study: the microfluidic device we designed to separate sperm based on rheotaxis and a simple passive microfluidic device. We compared the results with the density gradient centrifugation technique. Sperm attributes including concentration, morphology, viability and motility were assessed using related procedures. Statistical analyses were conducted using one-way analysis of variance. Results showed differences in sperm concentration, motility, morphology and vitality using different sperm separation techniques. The sperms separated using our microfluidic device demonstrated the highest motilities, normal morphology percentages and higher sperm vitality but significantly lower sperm concentrations. These findings suggest the potential of our microfluidic design in enhancing sperm quality. Our findings are in agreement with previous research, emphasizing the capability of microfluidics in enhancing sperm quality. Specifically, our designed microfluidic device exhibited exceptional efficacy in isolating highly motile sperm, a critical factor for successful fertilization.

  • Chapter 9 - Sperm Preparation

  • David Mortimer, Lars Björndahl, Karolinska Institutet, Stockholm, Christopher L. R. Barratt, University of Dundee, José Antonio Castilla, Roelof Menkveld, Stellenbosch University, South Africa, Ulrik Kvist, Karolinska Institutet, Stockholm, Juan G. Alvarez, Trine B. Haugen
  • Book:
    A Practical Guide to Basic Laboratory Andrology
    Published online:
    16 February 2022
    Print publication:
    10 March 2022, pp 194-217

  • Summary

    The physiology and basic principles of sperm washing and other preparative techniques are described, including the limitations and even dangers posed by some methods. Optimized methods for sperm preparation using direct swim-up from semen and density gradient centrifugation are provided as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on standardization and robustness, and minimizing the risk iatrogenic damage to the spermatozoa and avoiding errors. There are sections on selecting which method to use, dealing with atypical semen specimens, processing multiple specimens, and potential new technology for preparing human spermatozoa for use in assisted conception treatment.

  • 4 - Donor insemination

  • Edited by Paul Serhal, Caroline Overton
  • Book:
    Good Clinical Practice in Assisted Reproduction
    Published online:
    22 October 2009
    Print publication:
    01 July 2004, pp 86-99

  • Summary

    In the UK, strict adherence to guidelines laid down by the Human Fertilisation and Embryology Authority (HFEA) has made the recruitment of sperm donors an arduous task. In donor insemination, the protection of the recipient and potential offspring from sexually transmitted and other inheritable disorders is paramount. When compared with embryos, the cryopreservation of sperm is usually relatively crude. Density gradient centrifugation (DGC) is almost universally accepted as the superior sperm preparation method for assisted reproductive techniques, donor insemination being no exception. Donor insemination provides an excellent research model. Several authors have used the system to examine the influence of semen parameters and sperm function testing on success rates. Donor insemination also provides an excellent tool to examine other factors governing success, for example, timing of inseminations and subtle female factors as the male gametes are of reasonably standardized quality.