In the nob mouse, a mutation in nyctalopin results in a loss
of signal transmission from photoreceptors to depolarizing bipolar
cells (DBCs). We used immunohistochemical techniques to assess the
expression pattern of proteins found at either the photoreceptor
terminal or bipolar cell dendrites within the outer plexiform layer. We
labeled normal and nob retinas with antibodies against mGluR6,
PKC, G0α, bassoon, PSD-95, the α1F
subunit of voltage-gated calcium channels, trkB, and dystrophin. All
labeling patterns in nob and normal retinas were comparable to
those previously reported in mouse retina. Our results indicate that
the absence of nyctalopin does not disrupt the expression pattern of
other proteins known to be required for synaptic transmission.
