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fertilisation current

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Non-specific currents at fertilisation in sea urchin oocytes
Maria Laura De Simone, Lucia Grumetto, Elisabetta Tosti, Martin Wilding, Brian Dale
Journal:

Zygote / Volume 6 / Issue 1 / February 1998

Published online by Cambridge University Press:

15 January 2010, pp. 11-15
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Using the whole-cell voltage-clamp technique to clamp sea urchin oocytes we show that the fertilising spermatozoon triggers an inward current of −521 ± 56.7 pA (n = 8) at activation. Simultaneously, the plasma membrane depolarises and the conductance increases from 23.4 ± 1.4 to 40.6 ± 1.2 nS (n = 8). The I/V curve for the peak activation current is linear and the current reverses between 0 and + 20 mV, suggesting a non-specific ion current. Since injection of inositol triphosphate induced an inward current of −1062 ± 314 pA (n = 4), and the current was inhibited by preloading oocytes with the calcium chelator BAPTA, the non-specific activation current in sea urchin appears to be calcium dependent.