This paper studies the structure and preservational properties of lower bounded HNN extensions of inverse semigroups, as introduced by Jajcayová. We show that if $S^* = [ S;\; U_1,U_2;\; \phi ]$ is a lower bounded HNN extension then the maximal subgroups of $S^*$ may be described using Bass-Serre theory, as the fundamental groups of certain graphs of groups defined from the $\mathcal{D}$-classes of $S$, $U_1$ and $U_2$. We then obtain a number of results concerning when inverse semigroup properties are preserved under the HNN extension construction. The properties considered are completely semisimpleness, having finite $\mathcal{R}$-classes, residual finiteness, being $E$-unitary, and $0$-$E$-unitary. Examples are given, such as an HNN extension of a polycylic inverse monoid.

We investigate a locally full HNN extension of an inverse semigroup. A normal form theorem is obtained and applied to the word problem. We construct a tree and show that a maximal subgroup of a locally full HNN extension acts on the tree without inversion. Bass-Serre theory is employed to obtain a group presentation of the maximal subgroup as a fundamental group of a certain graph of groups associated with the D-structure of the original semigroup.