Currently, no effective treatment is available for trichinellosis, a zoonotic parasitic disease caused by infection with the genus Trichinella. Kaempferol (KPF), a dietary flavonoid, has been documented to have anti-parasitic effects and various medicinal uses. Thus, this study aimed to investigate the efficacy of KPF in preventing and treating the intestinal and muscular phases of trichinellosis in mice compared with albendazole (ABZ). To achieve this, mice were divided into six groups: negative control; positive control; KPF prophylaxis; KPF treatment; ABZ treatment; and a combination of ABZ and KPF. Parasitological, histopathological and immunohistochemical evaluations were conducted to assess the effectiveness of the treatments. The parasitological assessment involved counting small intestinal adult worms and encysted muscle larvae. Additionally, the histopathological evaluation used the haematoxylin and eosin staining method for intestinal and muscular sections and picrosirius red stain for muscular sections. Moreover, the immunohistochemical expression of the intestinal NOD-like receptor-pyrin domain containing 3 (NLRP3) was evaluated. The group treated with combined drugs demonstrated a statistically significant reduction in the count of adults and encysted larvae (P < 0.05), a remarkable improvement in the inflammation of the intestines and muscles and a decrease in the thickness of the larvae's capsular layer. Additionally, the highest reduction in NLRP3 expression was observed in this group. Based on this study, KPF shows promise as an anti-trichinellosis medication that, when taken with ABZ, has a synergistic impact by modulating inflammation and larval capsule formation.

This study investigated the effects of pretreatment with antioxidants, kaempferol, and zinc gluconate on serum biochemical changes and impairment in body weight gain following noise-exposure in Wistar rats. Thirty-five animals were evenly grouped into five cohorts: Groups II, III, IV, and V were exposed to noise stress, induced by exposing rats to 100 dB (4 hr/day) for 15 days, from days 33 to 48 after starting the drug treatments. Treatment with kaempferol and/or zinc mitigated noise-induced deficits in body weight gain, and levels of serum lipid and protein fractions. The combined treatment significantly (p < .05) decreased malondialdehyde concentration in kaempferol + zinc gluconate treated group, compared to the group administered deionized water + noise. This result demonstrates that biochemical dyshomeostasis and lipid peroxidation may be involved in the molecular mechanism underlying noise stress and the assortment of kaempferol and zinc gluconate produced an improved mitigating outcome in Wistar rats.

Kaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

Anthocyanin indexes, quercetin, kaempferol and myricetin may provide industry with potential new medicines or nutraceuticals. Velvetleaf (Abutilon theophrasti Medik) leaves from 42 plant introductions (PI) were analyzed for anthocyanin indexes while both leaves and fruit were used for quercetin, kaempferol and myricetin concentration analysis by reverse-phase high performance liquid chromatography. Leaf anthocyanin indexes ranged from 6.15 to 11.25 among PI. Leaf quercetin and kaempferol concentrations ranged from 1.50 to 4.79 mg/g and 0.43 to 2.17 mg/g, respectively. Fruit quercetin, kaempferol and myricetin concentrations ranged from 0.061 to 0.266 mg/g, 0.054 to 0.734 mg/g, and 0 to 35.87 μg/g, respectively. Significant differences in leaf weight were also observed. Significant correlations were found between several traits. This information regarding anthocyanin indexes, quercetin, kaempferol and myricetin concentrations will be useful for velvetleaf cultivar development. Breeders and other scientists could use this germplasm that contains high concentrations of anthocyanins, quercetin, kaempferol and myricetin to develop new medicines or nutraceuticals from an extremely useful weedy species.

Consumption of fruits and vegetables has been associated with cancer prevention; flavonoids are widely distributed in plant foods and considered to be the active ingredients. Quercetin and kaempferol are two of the most commonly found dietary flavonols, and have been reported to prevent cancer. We have previously reported that the isoflavone genistein and the flavone baicalein exert differential actions on the oestrogen receptor (OR) α in HepG2 cells. Because of the structural resemblance to both isoflavone and flavone, we examined the effects of these dietary flavonols on ORα– and ORβ–specific transactivations and their subsequent involvement in inducing MCF-7 cell death. In the present study, both quercetin and kaempferol were able to compete for OR binding in a cell-free system and were agonistic to ORα and -β expressed in HepG2 cells, while some additive effect was observed in the oestrogen response element (ORE)-driven transcription when 17β-oestradiol was co-administered. Since the bcl-2 promoter contained two ORE, and ORE-driven transcriptional activity and Bcl-2 mRNA expression were increased by treatment with 10 μm-quercetin or kaempferol, it is possible that quercetin and kaempferol might up-regulate Bcl-2 expression through OR transactivation in MCF-7 cells. Cell death ELISA assay performed on MCF-7 cells indicated that an increase of apoptosis occurred at 25 μm-, but not 10 μm-, quercetin or kaempferol. Indirectly the results suggest that OR activation is not sufficient to induce apoptosis and that apoptosis is induced despite an increase in Bcl-2 expression.