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1 results

  • Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery

  • B Levin, S L Redmond, R Rajkhowa, R H Eikelboom, M D Atlas, R J Marano
  • Journal:
    The Journal of Laryngology & Otology / Volume 127 / Issue S1 / January 2013
    Published online by Cambridge University Press:
    15 August 2012, pp. S13-S20
    Print publication:
    January 2013
  • Background:

    Chronic tympanic membrane perforations can cause significant morbidity. The term myringoplasty describes the operation used to close such perforations. A variety of graft materials are available for use in myringoplasty, but all have limitations and few studies report post-operative hearing outcomes. Recently, the biomedical applications of silk fibroin protein have been studied. This material's biocompatibility, biodegradability and ability to act as a scaffold to support cell growth prompted an investigation of its interaction with human tympanic membrane keratinocytes.

    Methods and materials:

    Silk fibroin membranes were prepared and human tympanic membrane keratinocytes cultured. Keratinocytes were seeded onto the membranes and immunostained for a number of relevant protein markers relating to cell proliferation, adhesion and specific epithelial differentiation.

    Results:

    The silk fibroin scaffolds successfully supported the growth and adhesion of keratinocytes, whilst also maintaining their cell lineage.

    Conclusion:

    The properties of silk fibroin make it an attractive option for further research, as a potential alternative graft in myringoplasty.