The effects of the 4.2 kya climatic event on northern Mesopotamia have been the subject of significant scholarly debate, with the notion of a megadrought that forced local populations to migrate attracting particular attention. Here, the authors analyse stable carbon (δ13C) and nitrogen (δ15N) isotopes in human tooth and bone samples to assess trends in subsistence practice at three sites in Syria before, during and after the presumed megadrought event. Despite the proximity of the sites, isotopic differences between them are more significant than diachronic change. Combined with other archaeological evidence, these results indicate a continuity in subsistence patterns, with no indication of disruption associated with the 4.2 kya event.