A pre-launch survey of broadleaf weeds was conducted to predict the weed management efficacy of a novel genetically engineered sugar beet with resistance traits for glyphosate, dicamba, and glufosinate. We targeted problematic broadleaf weed species prevalent in the sugar beet system, including kochia, common lambsquarters, Palmer amaranth, and redroot pigweed across sugar beet areas in Colorado, Nebraska, and Wyoming. The results revealed that a significant percentage of kochia populations in Colorado, Nebraska, and Wyoming exhibited resistance to glyphosate (94%, 98%, and 75%, respectively) and dicamba (30%, 42%, and 17%, respectively). Palmer amaranth populations had resistance frequencies for glyphosate and dicamba of 80% and 20% in Colorado and 20% and 3% in Nebraska, respectively. No resistance to the tested herbicides was identified in common lambsquarters or redroot pigweed. Glufosinate resistance was not identified for any species. Kochia and Palmer amaranth populations from Colorado and Nebraska exhibited glyphosate resistance primarily through 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) gene amplification. However, one glyphosate-resistant kochia population from Wyoming lacked EPSPS gene amplification, indicating the presence of an alternative resistance mechanism. We identified the previously characterized IAA16 G73N substitution in a dicamba-resistant kochia population from Nebraska. However, dicamba-resistant kochia populations from Colorado did not possess this substitution, suggesting an alternative, yet-to-be-determined resistance mechanism. The widespread prevalence of glyphosate and dicamba resistance, coupled with the emergence of novel resistance mechanisms, poses a significant challenge to the long-term efficacy of this novel genetically engineered sugar beet technology. These findings underscore the urgent need for integrated weed management (IWM) strategies that diversify effective herbicide sites-of-action and incorporate alternative weed management practices within cropping systems.

Potato is the third most important staple food crop globally following rice and wheat. In the United States, potato is grown on approximately 410,000 ha with a farm-gate value of US$1,032 million. In Canada, potato is grown on approximately 134,000 ha with a farm-gate value of US$235 million. The objective of this manuscript, compiled by the Weed Science Society of America Weed Loss Committee, was to estimate potato yield loss caused by weed interference. Potato yield data from weedy and weed-free plots (or plots with >95% weed control) was obtained from researchers working on weed management in potato in the United States and Canada or from published manuscripts from 2000 to 2018. Potato yield loss from weed interference was 12% to 61% when no weed management tactics were implemented. The average yield loss for all states/provinces (where data was obtained) due to weed interference was 44%. Weed interference would cause a farm-gate loss of approximately US$465 million and US$61 in the United States and Canada, respectively, if weeds are not controlled. These results indicate that weed management is critical for successful potato production, and that an ongoing need for research exists on weed management in this crop.

Kochia accessions (designated as KS-4A and KS-4H) collected from a corn field near Garden City, KS, have previously shown multiple resistance to glyphosate, dicamba, and fluroxypyr. These accessions were also suspected as being resistant to photosystem II (PS II) inhibitors. The main objectives of this research were to 1) confirm the coexistence of cross-resistance to PS II inhibitors (atrazine and metribuzin) applied PRE and POST, 2) investigate the underlying mechanism of PS II-inhibitor resistance, and 3) determine the effectiveness of alternative POST herbicides for control of these multiple herbicide–resistant (MHR) kochia accessions. Results from dose-response experiments revealed that the KS-4A and KS-4H kochia accessions were 23-fold to 48-fold resistant to PRE- and POST-applied atrazine and 13-fold to 18-fold resistant to POST-applied metribuzin compared to a known susceptible kochia accession (KS-SUS). Both accessions also showed putative resistance to PRE-applied metribuzin that needs to be confirmed. Sequence analyses of the psbA gene further revealed that all samples from the KS-4A and KS-4H kochia accessions had a Ser264Gly point mutation. A pretreatment with malathion followed by a POST application of atrazine at 1,120 g ha−1 or metribuzin at 630 g ha−1 did not reverse the resistance phenotypes of these MHR accessions. In a separate greenhouse study, alternative POST herbicides, including bicyclopyrone + bromoxynil; bromoxynil + pyrasulfotole; paraquat alone or in combination with atrazine, metribuzin, 2,4-D, or saflufenacil; and saflufenacil alone or in combination with 2,4-D effectively controlled the KS-4H accession (≥97% injury). To our knowledge, this research reports the first case of kochia accessions with cross-resistance to PRE-applied atrazine and POST-applied metribuzin. Growers should adopt diversified weed control strategies, including the use of competitive crops, cover crops, targeted tillage, and harvest weed seed control along with effective alternative PRE and POST herbicides with multiple sites of action to control MHR kochia seedbanks on their production fields.

There is a continual need for invasive plant science to develop approaches for cost-effectively benefiting native over nonnative species in dynamic management and biophysical contexts, including within predominantly nonnative plant landscapes containing only small patches of native plants. Our objective was to test the effectiveness of a minimal-input strategy for enlarging native species patches within a nonnative plant matrix. In Pecos National Historical Park, New Mexico, USA, we identified 40 native perennial grass patches within a matrix of the nonnative annual forb kochia [Bassia scoparia (L.) A.J. Scott]. We mechanically cut B. scoparia in a 2-m-wide ring surrounding the perimeters of half the native grass patches (with the other half as uncut controls) and measured change in native grass patch size (relative to pretreatment) for 3 yr. Native grass patches around which B. scoparia was cut grew quickly the first posttreatment year and by the third year had increased in size four times more than control patches. Treated native grass patches expanded by an average of 25 m2, from 4 m2 in October 2015 before treatment to 29 m2 in October 2018. The experiment occurred during a dry period, conditions that should favor B. scoparia and contraction of the native grasses, suggesting that the observed increase in native grasses occurred despite suboptimal climatic conditions. Strategically treating around native patches to enlarge them over time showed promise as a minimal-input technique for increasing the proportion of the landscape dominated by native plants.

Evolution of kochia biotypes resistant to multiple herbicide sites of action is an increasing concern for growers across the U.S. Great Plains. This necessitates the development of integrated strategies for kochia control in this region based on improved forecasting of periodicity and patterns of kochia emergence in the field. Field experiments were conducted near Huntley, MT, in 2013 and 2014, and in Manhattan and Hays, KS, in 2013 to characterize the timing and pattern of emergence of several kochia populations collected from the U.S. Great Plains’ states. The more rapid accumulation of growing degree days (GDD) resulted in a shorter emergence duration (E90–E10) in 2014 compared with 2013 in Montana. Kochia populations exhibited an extended emergence period (early April through mid-July). Among all kochia populations, in 2013, Kansas-Garden City (KS-GC), Kansas-Manhattan (KS-MN), Oklahoma (OK), and Montana (MT) populations began to emerge earlier, with a minimum of 151 cumulative GDD to achieve 10% cumulative emergence (E10 values) in Montana. The New Mexico-Los Lunas (NM-LL) population exhibited a delayed onset but a rapid emergence rate, while the North Dakota (ND) and Kansas-Colby (KS-CB) populations emerged over a longer duration (E90–E10 of 556 and 547 GDD, respectively) in 2013 in Montana. In 2013 at the two locations in Kansas, kochia populations exhibited a similar emergence pattern, with no differences in the time to initiate germination (E10), rate of emergence (parameter b), or duration of emergence (E90–E10). At Hays, KS, the GDD for E50 and E90 were less for ND compared with KS-MN and KS-GC local populations. In 2014 the KS-MN kochia population exhibited an early (ED10 value of 215 GDD) but a more gradual emergence pattern (E90–E10=526 GDD) in Montana. In contrast, OK and New Mexico-Las Cruces (NM-LC) populations had an early and a more rapid emergence pattern (E90–E10=153 and 154 GDD, respectively). Kochia in Montana exhibited two to four emergence peaks. This differential emergence pattern of kochia populations reflects the occurrence of different emergence “biotypes” and emphasizes the need to adopt more location-specific and diversified weed control tactics to manage kochia seedbanks.

Timing of weed emergence and seed persistence in the soil influence the ability to implement timely and effective control practices. Emergence patterns and seed persistence of kochia populations were monitored in 2010 and 2011 at sites in Kansas, Colorado, Wyoming, Nebraska, and South Dakota. Weekly observations of emergence were initiated in March and continued until no new emergence occurred. Seed was harvested from each site, placed into 100-seed mesh packets, and buried at depths of 0, 2.5, and 10 cm in fall of 2010 and 2011. Packets were exhumed at 6-mo intervals over 2 yr. Viability of exhumed seeds was evaluated. Nonlinear mixed-effects Weibull models were fit to cumulative emergence (%) across growing degree days (GDD) and to viable seed (%) across burial time to describe their fixed and random effects across site-years. Final emergence densities varied among site-years and ranged from as few as 4 to almost 380,000 seedlings m−2. Across 11 site-years in Kansas, cumulative GDD needed for 10% emergence were 168, while across 6 site-years in Wyoming and Nebraska, only 90 GDD were needed; on the calendar, this date shifted from early to late March. The majority (>95%) of kochia seed did not persist for more than 2 yr. Remaining seed viability was generally >80% when seeds were exhumed within 6 mo after burial in March, and declined to <5% by October of the first year after burial. Burial did not appear to increase or decrease seed viability over time but placed seed in a position from which seedling emergence would not be possible. High seedling emergence that occurs very early in the spring emphasizes the need for fall or early spring PRE weed control such as tillage, herbicides, and cover crops, while continued emergence into midsummer emphasizes the need for extended periods of kochia management.

Field studies were conducted from 1986 through 1989 to determine the feasibility of using clomazone in a winter wheat-corn-fallow rotation. Clomazone at 1.1 kg ai/ha did not control emerged downy brome in autumn nor did it control redroot pigweed and tumble pigweed that emerged after a spring application of paraquat at 0.6 kg ai/ha. However, clomazone plus atrazine at 0.6 plus 2.2 kg ai/ha provided weed control in winter wheat stubble from October to corn harvest 12 mo later. Corn yields from this treatment were equal to or greater than the conventional practice of applying atrazine plus glyphosate at 2.2 plus 0.4 kg ae/ha in the fall followed by a spring application of metolachlor plus dicamba at 2.8 ai plus 0.3 kg ae/ha. When kochia densities were high, clomazone plus atrazine controlled triazine-resistant kochia better than metolachlor plus dicamba. Atrazine at 2.2 kg/ha applied in autumn followed by clomazone at 0.8 kg/ha in April generally caused more corn injury than when the same rate of clomazone was applied in autumn with the atrazine. The percent of bleached corn plants varied with planter, year, time of application, and rate of clomazone applied. The optimum time to apply clomazone at 0.6 kg/ha was in autumn with atrazine. This treatment caused some corn bleaching but produced the highest corn yield over 3 yr. In a dry autumn and spring, less clomazone injury occurred with a planter equipped with row cleaners than those without. The injury pattern associated with planters was inconsistent when precipitation was average and no injury occurred in a wet autumn and spring.

Two greenhouse experiments were conducted to compare the growth of individual sulfonylurea-resistant and -susceptible kochia under noncompetitive conditions. Aboveground leaf and stem dry weight, and leaf area per plant were measured weekly 14 times, starting 14 d after planting. Data were fitted using the Richards function for shoot dry weight per plant, a polynomial exponential for leaf area per plant and splined function to calculate leaf area ratio, leaf weight ratio, and leaf/stem ratio. Absolute and relative growth rates, and net assimilation rate were derived from these functions. Growth and development of individual sulfonylurea-resistant and -susceptible kochia plants under noncompetitive conditions was the same. Final shoot dry weight and leaf area were unaffected by aceto lactate synthase enzyme differences in the kochia biotypes. However, more resources were partitioned to leaves than stems in resistant than in susceptible kochia. If competitive abilities of sulfonylurea-resistant and -susceptible kochia are different, it is not the consequence of differential growth and ontogeny of the two types of plants.

Imazethapyr, alone and in combination with other herbicides, was applied PPI, PRE, and POST to pinto beans to determine weed control and selectivity to the crop. All of the herbicides improved pinto bean yield as compared with the unweeded control. Imazethapyr applied PPI and POST provided excellent control of black nightshade, kochia, Russian thistle, prostrate pigweed, and redroot pigweed. Barnyardgrass control with imazethapyr ranged from 58 to 96% and increased to 98% or greater when imazethapyr was combined with metolachlor, pendimethalin, trifluralin, or EPTC. Pinto bean yield was not reduced from any herbicide treatment compared to the handweeded control.

Weed management in furrow-irrigated corn is challenging because of weed emergence associated with each irrigation event. Residual herbicides that provide extended in-season control of a broad spectrum of weeds would be beneficial to producers in this system. Field experiments were conducted in 2005 and 2006 in Yellowstone County, Montana, to evaluate KIH-485 for the control of velvetleaf, kochia, and wild buckwheat in furrow-irrigated corn. KIH-485 was applied at three rates (166, 209, and 250 g ai/ha) and two timings (PRE and POST) and compared to standard rates of S-metolachlor, acetochlor, and pendimethalin. All PRE treatments were applied alone, whereas POST treatments were combined with 1,261 g ae/ha of glyphosate. All rates of KIH-485 applied PRE controlled velvetleaf and kochia 88% or greater at 4 mo after planting (MAP). Wild buckwheat was controlled 89% or greater with the high rate of KIH-485 applied PRE, which was superior to control achieved with any other PRE herbicide treatment. Velvetleaf, kochia, and wild buckwheat were controlled 91% or greater when any herbicide treatment was combined with glyphosate. Corn treated with KIH-485 applied at 209 g ai/ha PRE produced yield that was similar to that produced by the weed-free control in both years.

Field experiments were conducted in grain sorghum at five locations in Kansas in 2009 and 2010, to evaluate the efficacy and crop safety of early- to mid-POST (EMPOST) and late-POST (LPOST) applications of premixed pyrasulfotole and bromoxynil (PYRA&BROM) in tank mix combinations with atrazine or atrazine plus 2,4-D ester or dicamba compared to bromoxynil plus atrazine. PYRA&BROM at 244 or 300 g ai ha−1 plus atrazine at 560 g ai ha−1 applied EMPOST controlled pigweed species (Palmer amaranth, tumble pigweed, and redroot pigweed), kochia, velvetleaf, common sunflower, ivyleaf morningglory, and common lambsquarters 93% or greater. Puncturevine control among three locations ranged from 85 to 99%. Control of most weed species was not improved by increasing PYRA&BROM rate from 244 to 300 g ha−1 or by tank mixing 2,4-D or dicamba with PYRA&BROM plus atrazine. However, ivyleaf morningglory control was improved at the LPOST timing by adding 2,4-D or dicamba at 140 g ae ha−1. In no instance did any PYRA&BROM treatment provide greater weed control than bromoxynil plus atrazine at 281 + 560 g ha−1 when applied EMPOST, but in most instances PYRA&BROM treatments were more effective than bromoxynil plus atrazine when applied LPOST. Generally, PYRA&BROM treatments were more effective when applied EMPOST than LPOST, especially when 2,4-D or dicamba was added. PYRA&BROM plus atrazine treatments caused foliar bleaching in sorghum within 7 ± 3 d after treatment, but recovery was complete within 3 to 4 wk and grain yields were not reduced. Tank mixing dicamba with PYRA&BROM and atrazine occasionally reduced visible crop response compared to PYRA&BROM plus atrazine. Our results indicate that PYRA&BROM plus atrazine with or without 2,4-D or dicamba selectively controls several troublesome broadleaf weeds in grain sorghum. Foliar bleaching of sorghum leaves can occur but the symptoms are transient, and grain yields are not likely to be reduced.