The impact of alternative aviation jet fuels and their properties on lean blowout (LBO) limits has recently raised several questions in the jet fuel area. There is a need for a detailed investigation of the impact of fuel properties on the LBO limit involving actual engine hardware. This study investigates the impact of a range of alternative aviation jet fuels with notable differences in physical and chemical properties and derived cetane number (DCN) on the LBO limit and their effects on key performance indicators. LBO performance results for ten different alternative fuels using a Rolls-Royce single-can Tay combustor are presented in this study. The study also assesses impact of different equivalence ratios and flow rates on LBO, with the aim of determining the impact of a certain range of operating conditions. The results are further analysed to determine the influence of fuel chemical and physical properties on the LBO limit. Finally, based on results in the above experiments, individual fuel properties are adjusted for subsequent experimental analysis of blended fuels. With this approach, 25 additional fuel blends are evaluated and presented, with an emphasis on varying the DCN. This study provides effective data and results to facilitate future fuel optimisation and reduce the risk of a negative performance of new fuels in gas turbines.