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1 results

  • Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard

  • Peter J. Verveer, Vincent T.G. Schoonderwoert, Denis Ressnikoff, Shane D. Elliott, Kiefer D. van Teutem, Tobias C. Walther, Hans T.M. van der Voort
  • Journal:
    Microscopy Today / Volume 26 / Issue 5 / September 2018
    Published online by Cambridge University Press:
    11 September 2018, pp. 12-19
    Print publication:
    September 2018
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  • Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-to-noise ratio, LSFM data are well suited for image deconvolution. Here we present the Huygens Fusion and Deconvolution Wizard, a unique integrated solution for restoring LSFM images, and show that improvements in signal and resolution of 1.5 times and higher are feasible.