Two new species of gentian, Gentiana wangchukii E. Aitken & D.G. Long sp. nov., endemic to Bhutan, and Gentianella urnigera E. Aitken & D.G. Long sp. nov., from East Nepal, Sikkim, Central and Northern Bhutan and Yunnan, China, are described. Their generic placement and differences from allied species are discussed. The new combination Lomatogonium himalayense (Klotzsch) E. Aitken comb. nov. is made and this name is applied to plants previously ascribed to L. carinthiacum (Wulfen) Reichenbach from the East Himalaya; the basionym Pleurogyne himalayensis Klotzsch is lectotypified and epitypified. The genus Tripterospermum is reviewed in the Flora of Bhutan area and two species are recognized: T. volubile (D. Don) Hara and T. nigrobaccatum Hara. Within T. volubile, two subspecies are recognized: subsp. volubile and subsp. longipes E. Aitken & D.G. Long subsp. nov. T. luteoviride (C.B. Clarke) Murata, in the past treated as a distinct species, is placed in synonymy of subsp. volubile.