Due to the lack of reliable yield monitor for sugarcane, production factors which impact and limit stalk yield within fields are not well-known. Thus, this study aims to evaluate whether canopy sensor technology is able to identify sugarcane biomass variability and whether obtaining other agronomic variable data can assist on biomass quantification. For that, forty targeted plots were allocated within two sugarcane-producing fields and data consisted on manual biometric evaluation, aboveground biomass measurement and canopy reflectance. As an ongoing experiment, only two evaluations were addressed (~0.3 and 0.5 m stalk height). On the earliest stage, canopy sensor readings were correlated to sugarcane biomass and their sensitivity to biomass variability was high. Further, data collected on the first evaluation was efficient in predicting biomass amount after 30 days. On the second, canopy sensor readings effectiveness to predict biomass was reduced. These findings suggest that crop canopy reflectance sensing is a useful approach to investigate sugarcane biomass spatial-variability within fields on early stages.