Thirty-four spedes of mosquitoes (Diptera: Culicidae) were collected at a series of human-bait catches designed to study evening crepuscular biting activity using the Nielsen crep unit, at a site at Peradeniya, Sri Lanka. Crepuscular biting profiles of seven species (Armigeres subalbatus, Aedes albopictus, Culex pseudovishnui, Cx. gelidus, Cx. fuscocephala, Cx. tritaeniorhynchus and Mansonia uniformis) were obtained. Modal periods for the onset of biting were analysed in six species: Cx. pseudovishnui, Cx. fuscocephala, Cx. gelidus, Cx. tritaeniorhynchus, Cx. quinquefasciatus and Ma. uniformis. Successions in the biting peaks and in modal onset of biting were seen in these species. Analysis of biting in Ar. subalbatus and Cx. pseudovishnui under high (clear sky) and low (cloud-overcast sky) light intensities showed earlier biting in both species under the latter conditions. The value of the Nielsen crep unit in making latitudinal comparisons is illustrated in Ma. uniformis, for which parallel data are available from Africa.