Observations, photographs, and video footage of a 337 cm total length female smalltooth sand tiger shark, Odontaspis ferox made at a depth of 348 m on the northern slope of San Ambrosio Island in the Desventuradas Islands (26°19.456′S 79°52.281′W) on 25 February 2013 represent not just the first record of this species in Chilean waters, but the first in the entire south-eastern Pacific Ocean, marking a tremendous range extension of this species. We also summarize the few known occurrences of this species along the Pacific and Atlantic coasts of South America.