As a fatigable part of industrial products, cable is a valuable research topic to predict the product lifetime. Since the cable fatigue is related to the bending radius in motion, this paper presents a cable motion capture and bending radius calculation method based on optical tracking system. In particular, a marker sorting algorithm is developed for further spline interpolation in cable motion tracking. The cable bending radius calculation precision is analysed as well. The Results show that the proposed method can successfully track cable motion with an acceptable error for cable lifetime prediction.