The study of colonizing and of dominant grass species is essential for
prairie conservation efforts. We sought to answer how naturalized Kentucky
bluegrass in the northern Great Plains has become successful in the last 20
yr despite its long history in the northern Great Plains. We tested for
evidence of geographical differentiation using flow cytometry and
microsatellite markers to ascertain the population genetics of Kentucky
bluegrass. Across all tested wild populations, high levels of genetic
diversity were detected along with moderate levels of structure. Mantel
tests of geographical patterns were not significant. Using clonal
assignment, we found two major clones that made up the majority of the
tested wild populations. When we compared the wild individuals to pedigree
cultivars, we found virtually no genetic overlap across all tests, which did
not support our hypothesis of developed cultivars contributing to high
genetic diversity in natural populations. Furthermore, DNA content tests
indicated a narrow range in ploidy in wild populations compared with lawn
cultivars, further supporting a hypothesis of divergence between wild and
pedigree cultivars. These results indicate the recent invasion of Kentucky
bluegrass in the northern Great Plains is not because of adaptation or
propagule pressure, but rather likely an environmental or land use
shift.