In late eighteenth-century Mexico City, Spanish colonials, particularly members of the urban middle and popular classes, performed a number of weddings and baptisms on puppies (which were wearing clothes or bejewelled collars) in the context of fandangos or dance parties. These ceremonies were not radical challenges to orthodoxy or conservative reactions in the face of significant economic, political, religious and cultural Bourbon reforms emanating from Spain. Employing Inquisitorial investigations of these ceremonies, this article explores the rise of pet keeping, the meanings of early modern laughter and the implications of the cultural and religious components of the Enlightenment-inspired Bourbon reforms in late colonial Mexico.

Fungi can be found in almost any environment, and play important roles in ecosystem processes such as nutrient cycling and degradation. Despite their importance, the vast majority of fungi have not yet been isolated and identified. Due to the difficulties inherent in culture-based methods, fungal ecologists have turned to community fingerprinting techniques, which utilise signal molecules to profile the fungal members of an environmental sample without culturing. Commonly used signal molecules include chitin, ergosterol, membrane lipids and nucleic acids. Several DNA-based fingerprinting methods have been successfully applied to fungal communities, including D/TGGE (denaturing/temperature gradient gel electrophoresis), SSCP (single-stranded conformational polymorphism), RISA (ribosomal intergenic spacer analysis), and T-RFLP (terminal restriction length fragment polymorphism). These techniques allow the fungal ecologist to rapidly profile fungal populations in an ecosystem, without the need for laborious culturing or cloning.