The population of the Critically Endangered Mariana crow Corvus kubaryi on the island of Rota, Commonwealth of the Northern Marianas Islands, has decreased dramatically in recent years. It is unclear to what extent negative practices by people, such as inappropriate land use or persecution of crows, have contributed to this decline. We conducted a public opinion survey to document ongoing practices towards the crows on Rota, to assess residents’ knowledge of and attitudes towards the birds, and to gauge potential responses to a government-instituted land incentive programme. Enumerators administered surveys in person during August 2011. Most of the 573 respondents were native Chamorro residents (75%) and more than half were landowners (62%). A majority of respondents (72%) considered environmental issues ‘very important’ and 76% knew of the Mariana crow's Critically Endangered status. Fewer respondents (55%) expressed concern about the bird going extinct. A number of respondents condoned shooting and chasing crows (17 and 52%, respectively), suggesting that residents may be harassing the birds. Chamorro landowners on the island were more likely to have negative attitudes towards the crows and to know people who persecute the crows than other island residents. Education was positively correlated with knowledge and concern about the crow and environmental issues, suggesting that new educational programmes on Rota may help improve residents’ attitudes towards the species. In addition, we recommend a revision of current land-use regulations and implementation of a monetary compensation programme for owners of crow nesting habitat to improve landowners’ attitudes and practices.