John Lightfoot's account of lichens in Flora Scotica was the first Scottish lichen Flora and as such it was novel in several respects: 1) it was published in English; 2) it drew on the knowledge and expertise of several key local collectors and treated lichens from alpine areas for the first time; 3) it made lichens accessible in providing Linnaean binomials, colloquial English, and frequently also Gaelic names, together with lively descriptions, details of ecology, and medicinal or traditional uses when these were known. Of the 117 taxa listed, 109 were classified in the genus Lichen, five in Byssus, two in Mucor and one in Fucus. Nineteen taxa were newly described, of which five are still in current use. John Lightfoot's life, work and botanical friendships are also briefly discussed.