The P3 amplitude is augmented by decrements in
target-probability, increments in the number of nontargets
preceding the target, and extensions of the interstimulus
interval (ISI). Each of these changes prolongs the target-to-target
interval (TTI) and, consequently, results attributed to
these factors might, at least partially, be accounted for
by the TTI. Recent research also indicates that the P3s
elicited by targets in one-, two-, and three-stimuli tasks
(in which the TTI remains invariant) are similar. However,
the TTI has not been examined systematically in previous
research. The present study had subjects listen to a randomized
ISI (0.5, 1, 2, or 4 s) version of the auditory oddball
task in which targets occurred after one, two, three, four,
or five nontargets. Event-related potentials were analyzed
based on ISI, sequential structure, and TTI. The study
examined sequence and ISI effects independent of TTI effects
and demonstrated that extensions of ISI affected N1 but
not P3 amplitude, extensions of TTI enhanced P3 amplitude
independent of sequential structure, and sequential structure
failed to influence P3 amplitude when TTI was controlled.