We present an assessment of the Southern Paraguayan Grasslands using Important Bird Areas (IBAs) located in a grassland landscape mosaic. Eleven IBAs in southern Paraguay were evaluated 10 years after their designation, using the BirdLife International method to assess the state, pressure, and response of these areas, during 2017 and 2018. Overall, the Pressure from ecosystem modifications led by fire, and fire suppression, agricultural expansion, and intensification due to farming and grazing have been identified as the major threats to IBAs. Regarding the State, 64% of the IBAs presented Very poor habitat quality to support grassland bird communities. The level of conservation Response was mostly negligible when considering conservation designation, management planning and conservation actions for the trigger species. Our results provide useful information to partners to reconsider these areas as IBAs as most of them no longer fulfill international requirements, we also highlight the importance of strengthening national policies to adequately protect natural grasslands.