Key mortality factors of sugar-cane top borer, Scirpophaga excerptalis Wlk. during the third brood were quantified on a susceptible sugar-cane variety, Co 1148 during 1981 and on susceptible CoJ 64, resistant CoJ 67 and collateral hoste, Sorghum halepense Peru, and Saccharum spontaneum L., during 1982 in Haryana (sub-tropical India). As artificial rearing of this borer is not possible, a methodology to have uniform age group at the beginning of the study by tagging the egg masses and the freshly attacked plants was developed. Results showed that parasitization of various stages and failure of neonate larvae to enter the midribs were the key mortality factors common to all the hosts. In S. halepense there was negative trend index (0.104) due to high mortality of larvae in all the instars. This revealed that it is not a suitable host for top borer. No predators were found to feed on any of the sub-imaginal stages of the borer due to the unique habits of the borer.