We conducted a primate survey in Samkos Wildlife Sanctuary, western Cambodia, during the wet season in 2009. We visually confirmed the presence of five out of six primate species thought to occur in the area: Bengal slow loris Nycticebus bengalensis, Indochinese silvered langur Trachypithecus germaini, pileated gibbon Hylobates pileatus, pig-tailed macaque Macaca leonina and long-tailed macaque Macaca fascicularis. We did not find any sign of the stump-tailed macaque Macaca arctoides and suggest it is absent in Samkos Wildlife Sanctuary and possibly from the Cardamom Mountains. We provide the abundance measures for each primate species; the three most abundant species were Bengal slow loris, Indochinese silvered langur and pileated gibbon. We propose Samkos Wildlife Sanctuary as a priority for primate conservation in Cambodia.