The amphidromous life cycle and morphological characteristics of Sicydiinae species allowthem to colonise tropical freshwater habitats from the river mouth to the upper reaches ofthe river basin. Otolith microchemistry of 62 Sicydium punctatum from thePérou River, Guadeloupe (French West Indies) was investigated with a femtosecond laserablation-inductively coupled plasma mass spectrometer (fs-LA-ICP-MS) in order toreconstruct individual life history and follow the possible movements of Sicydiumpunctatum after the recruitment. Sr:Ca fingerprint confirmed the amphidromouscycle of this species. Variations of Ba:Ca in some individuals indicated changes inenvironmental conditions after the recruitment in the river. Even if results did not allowconcluding to the specific origin of these variations, the hydrological regime and thebiofilm composition may have a non negligible influence on the Ba availability. Resultssupported the use of multi-elemental signatures in otoliths and highlight the need for alarge geographical and temporal sampling of Ba and Sr in freshwater systems for a betterunderstanding of amphidromous fish species.