The subjected status of the coloni equalled them to persons alieni iuris, as slaves were too, but they were still free. It made marriages with those not in this way subjected with regard to the transmission of status ‘unequal’. It implies that children follow the status of the mother. This ‘unequal marriage’ and its consequence was introduced by earlier laws. To prevent the children out of marriages of a colonus with a not subjected woman being not subjected, the senatusconsultum Claudianum was applied. That made children follow the status of the father. The abolition of the senatusconsultum led Justinian to introduce the faculty of estate owners to recall coloni from such marriages in order to prevent the loss of labour force. CJ 11.48.19 established that every colonus after thirty years of service was no longer alieni iuris and thus subjected to their estate owner, but free from his control over him and his property. Such coloni are called ‘free’ coloni (coloni liberi). They remained tied to the estate and had to render services and to pay poll tax, but could now fulfill public functions as no longer being of subjected status.

Darius I overcame rebellions and seized the throne of Babylon, but cuneiform scholarship continued and developed; religious practices did not change, nor did the great buildings on the citadel. The zodiac scheme came into use. The Achaemenid king took Babylonian royal titles and promoted the worship of Marduk for local purposes. Xerxes broke the continuity. Following an uprising, a purge led to the ending of many archives. The province of Babylon was divided in two. Subsequent Achaemenid kings continued to treat Babylon with reverence. Alexander the Great defeated Darius III, entered Babylon, retained the Persian satrap, and moved treasure from Susa and Ecbatana to Babylon. He was recognized as a god. Lack of sons at his premature death precipitated a civil war from which Alexander’s commander Seleucus emerged to take the throne jointly with his son Antiochus. The derelict ziggurat was demolished, but temples and rituals, chronicles and astronomical diaries, continued as before. Aramaic was widely used, and fewer texts were inscribed in cuneiform. Interest in the fall of Assyria and of the Babylonian empire is apparent in Greek literature. Famous scholars include Berossus and named astronomers. Parthians invaded and eventually ended the dynasty.