Some definitions of the term ‘lichen’ have often emphasized the role of the mycobiont as exhabitant in the symbiosis. Mastodia tessellata and Turgidosculum ulvae, both forming lichen-like associations with foliose algae, have traditionally defied that definition. In this study, we delve into the poorly known association of T. ulvae with Blidingia minima. Using four molecular markers (nrLSU, nrSSU, RPB1, mtSSU) we show that T. ulvae is a member of the family Verrucariaceae, closely related to the marine species Verrucaria ditmarsica. The presence of bitunicate asci and single-cell ascospores is confirmed. Our analysis of a fragment of the rbcL marker demonstrates that the photosynthetic partner belongs to B. minima, although relationships within this taxon remain unclear. Transmission electron microscopy allowed us to illustrate how T. ulvae interacts with Blidingia cells, and how haustoria in that species differ from those previously investigated in other marine lichen-forming fungi.

Ulva armoricana sp. nov. is described from the coast of Brittany, France, using the taxonomic criteria established by Bliding, Koeman and van den Hoek for the European species of Ulva. Morphological evidence strongly suggests that Ulva armoricana is conspecific with the free-living Ulva populations responsible for the ‘green tide’ phenomenon that occurs at some eutrophicated sites in North Brittany. Blades of U. armoricana are variable in shape, fragile, easily torn, and 30–55 μm thick in middle and apical regions; they differ from those of U. olivascens by their pale green colour and from those of Ulva lactuca, U. rotundata, U. gigantea, U. pseudocurvata and U. curvata by their crumpled texture that creates an irregular light-reflecting surface, and by the presence of tooth-like protuberances at the margin of the blade. U. armoricana differs from U. scandinavica in having longitudinal ribs containing closely packed bundles of rhizoids, except in the youngest thalli. U. armoricana can be discriminated from U. rigida by the polygonal (never rounded) cells in the mid-thallus region, and quadrangular cells arranged in small longitudinal rows in rapidly growing parts. Pyrenoid number, 1–2 (3–4), overlaps with that of other species, and shape of non-rhizoidal cells of the basal region in transverse sections, either oval or with tapered ends, appears not to be a good taxonomic character. Herbarium data indicate that the species has been collected in northern France since at least 1869, but was previously unrecognized.

Fine structure of vegetative cells, development and morphology of sporangia and general morphology of zoospores were studied for the first time in the genus Endophyton Gardner. Vegetative cells contained one parietal perforate chloroplast with 2–4 ulvophycean pyrenoids and transverse cell walls without plasmodesmata. Sporangial walls were formed by two distinct electron-dense and fuzzy layers which contained a contiguous electron-translucent, fibrillar material. Zoospores were naked, with an anterior dense aggregation of vesicles and a cup-shaped chloroplast with one eyespot. The flagellar apparatus showed 180° rotational symmetry and counterclockwise absolute orientation of its components. Microtubular roots had a cruciate pattern, in which d=2 and s=4 with 3/1 arrangement. Electron-dense bilobed terminal caps covered the proximal end of each basal body. These observations support the suggested close relationship of Endophyton to the allied genus Entocladia and other ulvalean algae, and reinforce the criteria for including the genus in the class Ulvophyceae, order Ulvales, family Ulvellaceae.