This paper aims to estimate Vessel Collision Frequency in the Singapore Strait. This frequency is obtained as the product of the number of Vessel Conflicts and the causation probability using the real-time vessel movement data from the Lloyd's Marine Intelligence Unit (Lloyd's MIU) database. The results show that the container carriers have the highest Vessel Collision Frequency while Roll-On Roll-Off (RORO) and passenger ships have the lowest frequency. Tankers cause the highest head-on collision frequency. In the Singapore Strait, the most risky overtaking area is between longitudes 103°48′E and 104°12′E. The most risky head-on area is between longitudes 103°50′E and 104°00′E while the majority of crossing collisions occur between longitudes 103°50′E and 104°12′E. The Vessel Collision Frequency is found to be 1·75 per year in the traffic lanes. Currently, westbound traffic in the Strait is more risky than eastbound traffic (the number of westbound collisions in July was 0·0991 while the number of eastbound collisions was 0·0470). Furthermore, the estimated Vessel Collision Frequency during the day is less than that at night. The results of this paper could be beneficial for the Maritime and Port Authority of Singapore to further enhance the navigational safety strategies implemented in the Singapore Strait.