Zoophycos-group burrows are prevalent elements of the post-Cambrian trace fossil record. Here we report the oldest specimens of Zoophycos from Lower Cambrian strata of the Lower Member Wood Canyon Formation in southeastern California. In addition to these being the oldest examples of this well-known trace fossil, the discovery of these specimens also reveals the presence of deposit feeding considerably earlier than has been suggested for the advent of this feeding style. This type of activity may have had a significant impact on sediment mixing during the Precambrian–Cambrian transition, though the rarity and shallow tier position of these specimens suggests otherwise.

Two types of pyrite framboids (PF, probably sulphate-reducing bacteria) have been found within the Zoophycos spreiten, hosted in the Guadalupian (Middle Permian) glaciomarine greywacke of the Westley Park Sandstone Member within the Broughton Formation from the southern Sydney Basin of southeastern Australia. They are composed of non-sheathed (PF1) and sheathed (PF2) sub-micron balls, respectively. Chemically, the sub-micron balls consist of iron, sulphur, carbon and oxygen. Both PF1 and PF2 occur in rhythmic alternation within the thick, light-grey and thin, dark-grey minor lamellae of Zoophycos spreiten. The framboids from the minor lamellae are highly abundant and occur in an orderly arrangement of equal density and in a good state of preservation. Within Zoophycos spreiten no homogeneous filling, fecal pellets, or any sign of re-exploitation of the minor lamellae have been recognized. No similar framboids have been observed outside Zoophycos spreiten. Therefore, the framboids are interpreted as the pyritized remains of microbial colonies within Zoophycos spreiten. The trace Zoophycos would be a multifunctional garden that may have been carefully constructed by the Zoophycos maker, where different microbial colonies were orderly and carefully planted and cultured within different minor lamellae. Further, it is proposed that the Zoophycos maker had a symbiotic relationship with microbial colonies on the mutual basis of food supply and redox conditions. The fact that the overlying spreiten cut the underlying ones indicates that the Zoophycos from the study area is of an upward construction. The rhythmic alternation of both the thick, light-grey and thin, dark-grey minor lamellae within Zoophycos spreiten may be suggestive of a gardening manner of the Zoophycos maker responding to the warm and cold changes, food supply in pulses and variations of sedimentation rate for planting and culturing microbial colonies under the conditions of a glaciomarine environment at the high latitudes.

A 425 ka long record from piston core GIK17925-3 taken in the northeastern South China Sea was used to study the environmental conditions controlling the distribution of the Zoophycos trace fossil. The 12 m long core offers a unique opportunity to study the response of the Zoophycos-producing animal to environmental variations over four glacial–interglacial cycles. The trace fossils show a strong glacial to interglacial variation in their abundance with a special preference for intervals with low sedimentation rates. Additional X-ray radiograph studies of piston cores from the Southeast Asian Marginal Seas show that the trace fossil Zoophycos is widespread in slope and deep-marine sediments, with the highest abundances encountered in low sedimentation rate settings. The preference of the Zoophycos producer for low sedimentation rates in a setting with strong seasonal fluctuations in food supply due to the shift between winter and summer monsoons, is interpreted to be the result of a cache-model behaviour, where food is collected during rich times and squirrelled away for poor times. Core GIK17925-3 also offers an opportunity to assess the impact of Zoophycos bioturbation on various palaeoenvironmental proxies. In this core, more than 30 % of the measured data points were more or less strongly affected by Zoophycos bioturbation. Together with the widespread occurrence this percentage indicates that Zoophycos may pose a serious threat to palaeoclimatic reconstructions in cores from low to moderate sedimentation rate sites.