Stress cues can affect the welfare of animals in close proximity and are possibly useful non-invasive indicators of the emitters’ welfare. To facilitate their study in murids, we tested whether rats’ stress odours could be collected and stored using an enfleurage-type technique. ‘Donor’ rats were individually exposed to a compound stressor (carried circa 75 m inside a novel container, then euthanised with rising carbon dioxide) while on blotting paper dotted with melted vegetable lard. These sheets were sealed, left at room temperature for 2-5 h, and then ‘bioassayed’ by a blind observer for their effects on conspecifics. Compared with control sheets (exposed to unstressed rats, to CO2 alone, or untreated), stress-exposed sheets significantly affected the unconditioned behaviour of 16 pairs of detector rats trained to enter an arena from their home cage to obtain sucrose. When used to line this arena, the stress-exposed sheets significantly increased: i) rats’ latencies to eat, to place front feet into, and to completely step into the arena and ii) shuttling movements between arena and home cage. These pilot data thus suggest that odours produced by stressed rats can be simply and successfully collected and stored for several hours, though certain potential confounds (eg urine volume) may conceivably be alternative explanations for the observed effects. Future work should control for urine volume, and assess whether fat is needed for optimal odour absorption by paper and for how long sheets can be stored at various temperatures. Much fundamental work is also still needed on the nature, functions, and sources of stress odours.

Introduction: Studies have demonstrated the presence of stress and post-traumatic stress among ambulance personnel, but no previous research has focused on the body's reaction in the form of the change in heart rate of ambulance staff in association with specific occupational stress.

Hypothesis: The purpose of this study is to investigate whether work as an ambulance professional generates prolonged physiological arousal that can be measured by heart rate in different situations.

Methods: Twenty participants carried a pulse-meter in the form of a wristwatch, which continuously measured and stored their heart rate 24 hours per day for a period of seven days. All ambulance alarms that occurred during the test period were recorded in journals, and the participants completed diaries and a questionnaire describing their experiences. The alarms were divided into different phases. Correlations between heart rate in the different phases were computed.

Results: Analysis of study data indicated a significant rise of heart rate unrelated to physical effort during an emergency alarm and response. This increased heart rate was noticed throughout the mission and it was not related to the length of experience the staff had in the ambulance profession. In addition, a non-significant trend suggested that alarms involving acutely ill children lead to an even higher increase in heart rate. In addition, this research showed that constant tension existed during sleep, while available for an emergency, indicated by a noticeable increase in heart rate during sleep at work compared to sleeping at home.

Conclusions: A rise in heart rate was experienced during all acute emergency missions, regardless of a subject's experience, education, and gender. Missions by themselves generated a rate increase that did not seem to correlate with physical effort required during an emergency response. This study shows that working on an ambulance that responds to medical emergencies is associated with a prolonged physiological arousal.

This is a descriptive study of the medical responses to the bombings by terrorists in Madrid on 11 March 2004. The nature of the event, the human damage, and the responses are described. It describes the: (1) nature and operations associated with the alarm; (2) assignment of responding units and personnel; (3) establishment and operations of casualty collection points; (4) medical transport and distribution of injured victims; (5) prioritization and command; (6) hospital care; (7) psychosocial care; (8) identification of the dead; and (9) police investigation and actions. Each of these descriptions is discussed in terms of what currently is known and the implications for future planning, preparedness, and response.