This article presents evidence of population movements in Thessaly, Greece, during the Early Iron Age (Protogeometric period, eleventh–ninth centuries bc). The method we employed to detect non-local individuals is strontium isotope analysis (87Sr/86Sr) of tooth enamel integrated with the contextual analysis of mortuary practices and osteological analysis of the skeletal assemblage. During the Protogeometric period, social and cultural transformations occurred while society was recovering from the disintegration of the Mycenaean civilization (twelfth century bc). The analysis of the cemeteries of Voulokaliva, Chloe, and Pharsala, located in southern Thessaly, showed that non-local individuals integrated in the communities we focused on and contributed to the observed diversity in burial practices and to the developments in the formation of a social organization.