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4 results

  • Ectopic autologous transplantation of ovarian tissue as a feasible technique to assess ovarian morphophysiology

  • Bárbara Rodrigues Nascimento, Guilherme Antonio de Gouvêa Lopes, Danielle Storino de Freitas, Paulo Henrique Almeida Campos-Junior
  • Journal:
    Zygote / Volume 30 / Issue 3 / June 2022
    Published online by Cambridge University Press:
    29 September 2021, pp. 416-418

  • The cryopreservation of murine ovarian tissue and its transplantation can be a promising technique for the preservation of fertility and an alternative for the future reconstitution of scientific valuable strains of mice. Accordingly, the aim of this study was to describe the entire surgical procedure for ovariectomy and dorsal subcutaneous autotransplantation in mice, and also some data about the efficiency of this procedure. Female C57Bl/6J mice (n = 18) were anaesthetised and bilaterally ovariectomized. After surgery, ovaries were autotransplanted in small subcutaneous pouches in the dorsal region of the forelimbs. The animals were inspected daily and, 23 days after transplantation, euthanasia and recovery of ovarian tissues were performed. Postoperative recovery, oestrous cyclicity, and folliculogenesis progression were evaluated. At 23 days after transplantation, the recovery of the ovaries was feasible, all classes (primordial to antral) of follicles were observed. Additionally, satisfactory efficiency rates were obtained, with 100% of anaesthesia survival rate, survival, graft recovery, folliculogenesis progression and oestrous cyclicity. In general, this short article describes ovarian ectopic autologous transplantation as an effective technique for maintaining rodent oogenesis and endocrine ovarian function. Even more broadly, we can still assume that the application of this technique may reduce the number of breeding matrices and experimental animals in the near future.

  • Chapter 26 - ART and oocyte donation in cancer survivors

  • from Section 6 - Fertility preservation strategies in the female: ART
  • Edited by Jacques Donnez, Université Catholique de Louvain, Belgium, S. Samuel Kim, University of Kansas
  • Book:
    Principles and Practice of Fertility Preservation
    Published online:
    04 February 2011
    Print publication:
    03 February 2011, pp 310-327

  • Summary

    Over the last several decades, survival rates for childhood cancer have steadily increased. Spermatogonial stem cells are responsible for the continual production of spermatozoa throughout adult life. Autotransplantation is considered more acceptable than allotransplantation or xenotransplantation, although both of the latter have been used successfully in mouse models. Spermatogenesis in vitro from biopsied germ cells is considered to be an excellent alternative for pre-pubertal boys with malignancies, particularly of hematopoietic origin, who carry a risk of relapse after transplantation. Despite significant advances in spermatogonial cell biology and subsequent fertility management, malignant contamination remains one of the main concerns surrounding autologous transplantation. The potential for transferring tumor cells within cryopreserved and subsequently cultured and/or expanded testicular tissue back into the patient is of paramount concern. Children most at risk of transmitting cancer cells include those with a haematological malignancy such as acute leukemia.

  • Chapter 11 - Fundamental cryobiology of reproductive cells and tissues

  • from Section 3 - Reproductive biology and cryobiology
  • Edited by Jacques Donnez, Université Catholique de Louvain, Belgium, S. Samuel Kim, University of Kansas
  • Book:
    Principles and Practice of Fertility Preservation
    Published online:
    04 February 2011
    Print publication:
    03 February 2011, pp 129-144

  • Summary

    This chapter focuses on female fertility preservation procedures because of their complexity and peculiarities. Ovarian failure leads to the impossibility of childbearing apart from other problems related to the menopause, such as vasomotor, skeletal or cardiovascular alterations. Early menopause and infertility are two of the main consequences for patients treated with gonadotoxic agents. Gonadotoxicity, a decrease in ovarian activity, depends on several factors, including the age of the patient; the initial status of the ovaries; the treatment applied and cumulative doses; and the type of agent used. Ovarian tissue freezing for later autotransplantation is alternative for fertility preservation in women with oncological or non-oncological diseases. Any patient with a high risk of premature ovarian failure is a possible candidate for fertility preservation. Oocyte and ovarian tissue cryopreservation are useful as they overcome some of the disadvantages, ethical concerns and legal restrictions related to embryo cryopreservation.

  • Chapter 40 - Psychological issues of cancer survivors

  • from Section 10 - Ethical, legal and religious issues with fertility preservation
  • Edited by Jacques Donnez, Université Catholique de Louvain, Belgium, S. Samuel Kim, University of Kansas
  • Book:
    Principles and Practice of Fertility Preservation
    Published online:
    04 February 2011
    Print publication:
    03 February 2011, pp 467-478

  • Summary

    Fertility preservation is now recognized as the most essential quality of life issue in young cancer survivors. This chapter discusses three urgent and critical problems involved with ovarian tissue cryopreservation and transplantation (cryoin-jury, ischemic tissue damage, cancer cell transmission). The risk of cancer cell transmission is a serious safety issue related to ovarian autotransplantation in cancer patient. There are three strategies, at least in theory, to mature follicles in frozen stored ovarian tissue: autotransplantation; xenotransplantation; and in-vitro culture. Recently, significant progress has been made in immature follicle culture techniques. As an alternative to ovarian tissue transplantation, whole ovary transplantation has been explored. In theory, whole intact ovary transplantation with vascular anastomosis can restore the full function of the ovary. The main challenge of whole ovary transplantation for fertility preservation is the development of effective cryotechnology for the whole organ.