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basal root thickness

Chinese Journal of Agricultural Biotechnology (1)

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Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice
Liu Li-Feng, Zhang Hong-Liang, Mu Ping, Qu Yan-Ying, Li Zi-Chao
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 4 / Issue 3 / December 2007

Published online by Cambridge University Press:

13 February 2008, pp. 199-205

Print publication:

December 2007
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The development of near-isogenic lines (NILs) is an important fundamental step in the cloning of quantitative trait loci (QTL) and molecular marker assisted breeding. In the present study, NILs for two major QTLs of basal root thickness (BRT) and 1000-grain-weight (TGW) were obtained by molecular marker-assisted selection (MAS) through foreground selection for target QTL and background selection in three backcross generations (BC1F1, BC2F1 and BC3F1). After phenotypic evaluation in the BC3F2 generation, nine BRT QTL-NILs with BRT ranges of 1.07–1.16 mm (6.11–15.18% greater than the recurrent parent) and an average recovery ratio of genetic background (RRGB) of 97.22%, and 11 TGW QTL-NILs with ranges of 21.25–26.25 g (7.05–32.16% greater than the recurrent parent) and 95.97% of RRGB, were selected.