A new record on the occurrence of Neobythites multistriatus which hitherto had not been recorded from the Bay of Bengal is reported. Two specimens measuring 160 and 180 mm standard length were collected from the landings of a deep-sea trawler by-catch at depths of 200–250 m off the Tuticorin coast in the Gulf of Mannar (part of the Bay of Bengal) during March 2010. This is the first record of Neobythites multistriatus from the Bay of Bengal. The specimen is described and figured.