Serranus hepatus is a bycatch species in the Mediterranean trawl fishery for which knowledge on biological features such as otolith morphology is generally poor in the Mediterranean Sea. In the present study, the weight–length relationship, age, growth and ten otolith morphometric variables of this species were investigated in two distant areas, the Eastern Ionian and southwestern Aegean Seas. Isometric growth in weight was defined in both areas. Growth parameters also did not differ between the two study areas. Six of the otolith variables (radius, otolith length, otolith width, otolith area, perimeter and ellipticity) showed a significant relationship with size without significant differences between the two areas. Significant differences between the two areas were only defined for five of the morphometric variables (otolith length, width and area being the most important). Further studies to discriminate between potential stocks of S. hepatus seem to be necessary, information that is needed for stock identification, stock assessment and fisheries management.