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1 results

  • Cryo-Confocal Imaging for CLEM Mapping in Brain Tissues

  • Connon I. Thomas, Nicolai T. Urban, Ye Sun, Lesley A. Colgan, Xun Tu, Ryohei Yasuda, Naomi Kamasawa
  • Journal:
    Microscopy Today / Volume 29 / Issue 5 / September 2021
    Published online by Cambridge University Press:
    24 September 2021, pp. 34-39
    Print publication:
    September 2021
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  • In correlative light and electron microscopy (CLEM) workflows, identifying the same sub-cellular features in tissue by both light (LM) and electron microscopy (EM) remains a challenge. Furthermore, use of cryo-fixation for EM is desirable to capture rapid biological phenomena. Here, we describe a workflow that incorporates cryo-confocal laser scanning microscopy into the CLEM process, mapping cells in brain slices to re-image them with serial section scanning electron microscopy (ssSEM) array tomography. The addition of Airyscan detection increased the signal-to-noise ratio (SNR), allowing individual spines in thick frozen tissue to be visualized at a sufficient spatial resolution, providing a new tool for a CLEM approach to capture biological dynamics.