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cumulus oocyte complex

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Chapter 4 - Oocyte Pick-Up for IVF
from Section 1 - Starting a New Laboratory and Training Protocols
- By Alison Campbell, Caroline Drew, Kathryn Berrisford, Louise Best
Edited by Markus H. M. Montag, ilabcomm GmbH, St Augustin, Germany, Dean E. Morbeck, Kindbody Inc, New York City
Book:

Principles of IVF Laboratory Practice

Published online:

07 August 2023

Print publication:

24 August 2023, pp 20-29
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Summary

Oocyte pick-up is the process whereby oocytes are microscopically identified and pipetted from follicular fluid aspirates and placed into a culture environment prior to in vitro fertilization (IVF). This chapter sets out the laboratory operating procedure for oocyte pick-up and considers practical training methods and competency assessment. From the outset, it is important for embryologists or technicians to be aware that changes in the wider environment of a gamete or embryo can affect its immediate culture environment. The external environment includes not only the space in which the pick-up is performed – the flow hood, which much provide stable temperature and pH maintenance for the oocytes – but also the consumables and culture media used. Task-based training leading to competency should follow a robust pathway. Oocyte pick-up is a critical process and, as such, should be performed effectively and reproducibly by the embryologists within the team.

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Chapter 4 - Oocyte Pick-Up for IVF

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Chapter 4 - Oocyte Pick-Up for IVF

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