Seventeen chondrichthyan species were caught in the Cantabrian Sea (southern Bay of Biscay) during a multidisciplinary survey carried out in the Avilés canyon system in May 2011. This survey provided the first records of three species (Galeus murinus, Neoraja iberica, and Neoraja caerulea) in these waters, and a further record of Rajella kukujevi. To confirm the identity of these species, the cytochrome c oxidase subunit I (COI) of the specimens was sequenced. Genetic analyses revealed that the DNA sequences of the two Neoraja species were identical in all the specimens analysed. Morphometric analyses, based on 40 characters, showed 3.66% dissimilarity between the two species. The morphometric character that contributed most to this discrepancy was disc width.