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Addressing important problems and processes in the assessment of substance use disorders (SUDs), this chapter reviews the types of information necessary for a formal diagnosis of a DSM-5 SUD. In addition, the chapter discusses seven assessment areas that must be examined if a given case is to be well evaluated. These include clinical history, strength of dependence syndrome, impaired volitional control, craving, neuroadaptation, negative consequences and pathological patterns, and stage of change. The origin and research background of each of these constructs are briefly outlined, a favored assessment tool for each is reviewed in detail, and alternative measures are also suggested. The assessment of SUDs is a multidimensional, complex process for which many excellent tools have been, and are still being, developed.