The incidence of the American serpentine leafminer, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) was studied in cotton-growing tracts of Tamil Nadu State, India. The incidence was higher on summer than on winter cotton and the cropping pattern was found to have an impact on the incidence of the leafminer on cotton. Biological studies on L. trifolii conducted on cotton, Gossypium hirsutum L., tomato, Lycopersicon esculentum Mill., and cowpea, Vigna sinensis (L.) Walp., revealed that its life cycle varied with different hosts. The duration of the pupal stage of L. trifolii was the longest in the leafminer's life cycle. Lycopersicon esculentum was the preferred host—females of L. trifolii survived the longest and had the highest fecundity on this host. Sex discrimination was possible at the pupal stage, on the basis of size; female adults were found to emerge from larger-sized pupae and males from smaller ones.
