Field bindweed, a member of the Convolvulaceae family, is a problematic perennial weed in cotton fields and orchards in northwest China. The species exhibits strong seed dormancy, causing delayed germination. A clear understanding of the mechanisms involved in alleviating seed dormancy is important for effective plant propagation and successful management of field bindweed. Experiments were conducted to investigate seed germination and radicle growth of field bindweed by breaking seed dormancy using mechanical scarification, sulfuric acid, hot-water scarification, cold stratification, and chemical treatment. Chemical treatments (gibberellic acid or potassium nitrate) had no effect on breaking seed dormancy, whereas mechanical scarification (sandpaper and blade) resulted in 92% to 98% seed germination, indicating that seed dormancy of field bindweed was mainly due to the presence of a hard seed coat. Seeds pretreated with 80% sulfuric acid for 15 to 60 min or 98% sulfuric acid for 15 to 30 min had germination rates above 80%, and soaking seeds in 70 C water for 4 to 16 min or in boiling water for 5 to 20s were effective in breaking seed dormancy but had no effect on the radicle growth of field bindweed. Cold stratification at 5 C for 2 to 8wk partially accelerated seed dormancy release, resulting in 53% to 67% seed germination. Results indicated that field bindweed could potentially form a persistent soil seed bank with physically dormant seed; therefore, strategies for eliminating seed production should be adopted.

Physical dormancy of Robinia pseudoacacia seeds makes it a challenge for scientists and forest managers to obtain a homogeneous germination for larger seed samples. Water imbibition of the seeds can be achieved through manual piercing of the seed coat, but this method remains time consuming and heterogeneous. We tested several ecologically friendly methods to break seed dormancy, including manual pin puncture, water soaking, oven dry-heating (two temperatures) and sanding. Sanding was performed using an automatic grinder to control shaking duration (three durations) and get a homogeneous scraping of the coat. All methods, except dry-heating, resulted in successful dormancy breaking; water soaking was the least efficient method, attaining 57% germination. Sanding proved to be as efficient as puncturing (97%) but long duration sanding (10 or 15 min) could damage cotyledons, which would impede further development of the plant. Short-time sanding (5 min) proved to be the best method to reach high total germination and healthy (undamaged cotyledon) seedlings, and was successfully applied to 500 seeds. The reference puncture method and the automatic sanding were also tested on seeds of nine Fabaceae species and proved to be efficient for some species. Automated sanding can thus be used as a standard to break physical dormancy of black locust or other Fabaceae seeds to allow further comparative studies of plant populations or genotypes.

A clear understanding of the mechanisms involved in releasing seed dormancy is important for effective plant conservation and regeneration of desert species. The dormancy types and dormancy release mechanisms of 19 Fabaceae species from four cold deserts in Xinjiang province, China, were studied. An imbibition experiment was performed to determine the presence or absence of physical dormancy. Other treatments included mechanical scarification, sulphuric acid scarification and simulation of summer temperatures typically encountered in the field. High summer temperature treatments included a maximum soil temperature of 80°C, 65°C and 40°C, conducted under dry and wet conditions. The results showed that all the species had orthodox seeds. Caragana korshinskii, Caragana intermedia, Caragana microphylla and Onobrychis taneitica seeds were not dormant. Glycyrrhiza uralensis, Ammodendron bifolium, Vicia costata and Eremosparton songoricum seeds had combinational dormancy (PY+PD) whereas the other 11 species had physical dormancy. The mechanical scarification and sulphuric acid scarification treatments were significant to break dormancy (>80%). Melilotus suaveolens and Oxytropis sp. seeds were temperature-insensitive. Sophora alopecuroides, S. salsula, E. songoricum, A. bifolium and Cassia tora seeds were wet-heat sensitive. Glycyrrhiza glabra seeds were dry-heat sensitive, while those of Halimodendron halodendron and Astragalus lehmannianus were sensitive to both dry and wet heat. Although high temperatures, typical of summer, promoted the breaking of physical dormancy, the proportion of seeds of which dormancy was broken differed among species. These differences were indicative of the conditions in which the species are found naturally. Species may have different adaptations that promote favourable regeneration. Given suitable temperature and moisture conditions similar to those in their natural environment, most species were capable of regeneration.

The endogenous ABA contents of dormant and nondormant barley grains were determined following application of different compounds to break dormancy. The chemicals used for breaking of dormancy in intact dormant grains were weak and strong acids, alcohols, hydrogen peroxide, cyanide, nitrate, salicylic acid, gibberellic acid and fusicoccin. The dormancy-breaking compounds could be classified into two major groups: compounds that caused a decrease in endogenous ABA (class I) and compounds which did not affect endogenous ABA (class II). Class I compounds included gibberellic acid, ethanol, hydrogen peroxide, nitrate, salicylic acid; class II compounds were fusicoccin, acid (H2SO4), sodium azide, n-caproic acid. In addition, these dormancy-breaking compounds were able to stimulate the germination rate when applied to embryos isolated from dormant grains. The concentrations necessary for stimulation of germination of isolated embryos were much lower than the concentrations for breaking the dormancy of intact grains. After embryos were isolated from dormant grains and incubated in water, ABA was determined in both embryos and in the incubation media. The class I compounds stated above also reduced ABA content in the incubation medium of isolated embryos, while class II compounds had no effect on ABA content of the medium. External application of ABA could overcome the effect of dormancy-breaking compounds of class I but not of class II. The results suggest that in the presence of the agents belonging to class II, ABA responsiveness of isolated embryos from dormant grains is decreased, compared to nontreated embryos.

Protein synthesis in embryonic axes of Norway maple (Acer platanoides) was studied in seeds stratified at 3 or 15°C. At 3°C stratification, germination of seeds started between 6 and 7 weeks; at 15°C stratification germination did not occur. During imbibition of embryonic axes undergoing cold and warm stratification many new proteins became visible. Two of these proteins may be connected with the release from dormancy at 3°C. In seeds stratified at 15°C, these proteins occur in low amounts or are completely absent.

Features of chemicals which break seed dormancy and the mechanisms of their action are discussed with reference to results obtained with red rice (Oryza sativa) and other relevant research. The significance of chemical structure, including the nature and location of functional groups, is considered, and the fact that similar structures can elicit different biochemical responses is illustrated with examples. As yet it is unclear whether changes in cell pH, highly correlated with the uptake of, and metabolism to, weak acids, and which some consider a possible trigger or marker in dormancy breaking, are causative, consequential or merely correlative. Uptake and metabolism of dormancy-breaking compounds is, or can be, very rapid and the importance of establishing the time course of dormancy-breaking, as opposed to germination events, is emphasized.

Introduction. The emergence of P. caerulea L. seeds was studied for the first time, in order to obtain useful information for cultivation of this plant with ornamental, edible and medicinal values. Materials and methods. Different treatments (aril removal, mechanical and chemical scarification and combinations of them) were performed on fresh seeds before sowing. The treatments simulated different paths that the seeds may follow in nature in this ornithochorus species, those which small growers could reproduce before cultivation. Seed emergence was recorded periodically for 16 months in a greenhouse. Seed emergence initial time, percentage and speed were calculated. Results and discussion. Seeds immersed in hydrochloric acid (chemical scarification) did not germinate, but the rest of the treatments were successful. Emergence percentages were low in most of the treatments and speed was slow in all of them. The minimum seed emergence initial time was 25 days. The curve of the accumulated number of seeds emerged followed an oscillating sigmoid pattern. Conclusion. It is suspected that dormancy exists, which will be dealt with in another paper.

Introduction. L'insuffisance en froid hivernal dans les zones de plaine au Maroc constitue un facteur limitant de la production du pommier. Nous avons évalué les effets du cyanamide hydrogène (Dormex), seul ou additionné d'huile minérale, et de l'acide gibbérellique sur le débourrement de pommiers, dans les conditions climatiques de plaines au sud du Maroc. Matériel et méthodes. Les expérimentations ont porté sur la variété `Dorsett Golden' connue pour ses faibles besoins en froid. Un dispositif en bloc aléatoire complet avec quatre répétitions a été utilisé. Les produits testés ont été le Dormex appliqué soit seul à (1,5, 2,5 et 4) % du produit formulé, soit à 0,5 % en association avec de l'huile minérale à (2 ou 4) %, et l'acide gibbérellique à 460 mg $\cdot$ L-1. Les arbres témoins ont été traités à l'eau. L'évaluation a porté sur le débourrement, la floraison, la nouaison, la croissance végétative et la production. Résultats. Les substances chimiques ont avancé la date de débourrement et en ont amélioré le taux de même que celui de floraison, et ils ont favorisé la croissance végétative. Le rendement et le calibre des fruits ont également été améliorés de manière significative. Conclusion. Le Dormex, seul ou combiné à de l'huile minérale, et l'acide gibbérellique ont avancé et regroupé le débourrement tout en améliorant le calibre et la production en fruits du pommier `Dorsett Golden'. L'efficacité du mélange [ Dormex + huile minérale]associée à son moindre coût conduisent à recommander ce produit pour pallier le manque en froid hivernal dans les zones de plaine du Maroc caractérisées par un hiver doux.

Introduction. For some species such as P. armeniaca, overcoming dormancy may be problematic when cold requirements are not satisfied. Moreover, the different positions of flower buds on the tree canopy and on the apical and basal portions of twigs can influence their development. Sampling problems have not been extensively studied. Therefore, the effect of different sectors (west and east), of different heights (top and bottom) of the canopy and of twig portions (medium-apical and medium-basal) on the break of dormancy in the apricot tree was evaluated. Attention was also focused on floral anomalies in relation to the flower bud position on the twig, in order to identify the least variable portion of twigs and thus provide useful information for optimization of periodic bud sampling. Materials and methods. Two cultivars of P. armeniaca with different chilling requirements were chosen: `San Castrese' and `Orange Red'. The end of endo-dormancy was evaluated from December to blooming time by traditional biological approaches, such as increase in flower bud fresh weight and evolution of the bud phenological stage, before and after 7 d under forcing conditions. In addition, the percentage of floral anomalies was considered in relation to the flower bud position on the twig. Results and conclusion. The results showed that canopy and twig positions exerted different effects on the breaking of the flower bud endo-dormancy, and some practical applications can be drawn from them for the best sampling design inside the tree structure: the west orientation of the canopy and the medium-apical portion of the twig were the best positions to obtain an earlier active growth of buds, allowing the prediction of endo-dormancy breaking. The analysis of flower anomalies showed considerable differences depending on the position of the bud on the twig. High percentages of anomalous buds were observed in the medium-basal portion of the twig, which could distort the results on the evaluation of overcoming dormancy, due to a weaker growth trend of flower buds.

Introduction. L'insuffisance en froid hivernal dans les zones de plaine au Maroc constitue un facteur limitant à la production du pommier. Les effets du dinitro-orthocrésol (DNOC), produit connu pour lever la dormance chez d'autres arbres fruitiers, ont été évalués vis-à-vis du débourrement de pommiers `Golden Delicious' cultivés dans de telles conditions climatiques. Matériel et méthodes. Un mélange de DNOC à 0,15 % et d'huile de pétrole utilisée a 3 % ou 5 % a été appliqué à des pommiers, à deux dates différentes. La production des arbres ainsi traités a été comparée à celle d'arbres témoins non traités. L'évaluation des traitements a porté sur les taux de débourrement des bourgeons végétatifs et floraux, la floraison, la nouaison, la croissance végétative et la production. Résultats. La solution utilisée a avancé la date de débourrement et amélioré de façon significative les taux de débourrement des bourgeons végétatifs et floraux, la croissance végétative, ainsi que le rendement et le calibre des fruits. Conclusion. Le mélange [DNOC + huile du pétrole] a permis d'avancer et de regrouper le débourrement de l'ensemble des bourgeons et a amélioré le calibre et la production du pommier `Golden Delicious'. Ce mélange pourrait donc être utilisé pour pallier le manque en froid hivernal des zones de plaine du Maroc, caractérisées par un hiver doux.