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ferric uptake regulator (fur)

Annales de Limnologie - International Journal of Limnology (1)

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Iron stress and genetic response in cyanobacteria : fur genes from Synechococcus PCC 7942 and Anabaena PCC 7120
J. A. Hernández, M. Artieda, M. L. Peleato, M. F. Fillat, M. T. Bes
Journal:

Annales de Limnologie - International Journal of Limnology / Volume 38 / Issue 1 / March 2002

Published online by Cambridge University Press:

15 March 2009, pp. 3-11

Print publication:

March 2002
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Fur (Ferric uptake regulator) is a DNA-binding protein which represses the transcription of iron-regulated promoters using Fe2+ as a cofactor. In Gram negative bacteria, fur controls the expression of toxins and virulence factors when facing iron-stress. Iron is a key element limiting the growth and proliferation of phytoplankton. We have cloned the fur-gene from the cyanobacterium Synechococcus PCC 7942, applying the polymerase chain reaction (PCR) technique. The DNA fragments amplified encodes a Fur-type protein, which conserves the main motifs found in the family of Fur proteins. Recombinant Fur protein was successfully overproduced, reaching about 15 % of the total protein content. Also, a Fur protein was identified in the filamentous, nitrogen-fixing cyanobacterium Anabaena PCC 7120.