Field experiments were conducted from 1989 through 1991 to determine the effect of DPX-PE350 applied postemergence over-the-top on cotton yield and fiber quality. DPX-PE350, at rates ranging from 50 to 280 g ae ha−1 applied to cotton in the VC to R6 growth stages, had no adverse effect on seed cotton yield, micronaire, fiber length, fiber length uniformity, or fiber strength. Cotton injury was 10% or less in all experiments.