Following a precultivation with pedospheric nitrogen nutrition,
nitrate or ammonium solutions were supplied to
the shoots of Ricinus plants by spraying (during the experimental
period) resulting in an increase of biotic/organic
and abiotic/inorganic particles on the surface, which significantly
increased wetting of the leaf surfaces. The
distribution of particles on the surface of sprayed leaves, in particular
crystals around and in stomata, indicated
the possible entry of nutrients via thin water films through the
stomatal pores in addition to diffusion through the
cuticle. Ammonium was taken up more readily than nitrate by the
foliage, but both at relatively low rates which
caused N limitation. Interestingly, the inorganic N, both in the
form of nitrate and even ammonium, was entirely
assimilated in the shoots; phloem transport of inorganic N to
the root was negligible. The flows of malate, and
the acidification of the apoplastic washing solution of leaves in
ammonium-sprayed plants pointed to the role of
metabolism of malate and excretion of protons in maintaining pH
during ammonium assimilation in the shoot.
Ammonium-sprayed plants incorporated the N in the same amounts in
shoots and roots, only 38% of the shoot-borne N being recycled in the
xylem. In nitrate-sprayed plants the root was not only favoured in N partitioning,
but even a net export of previously incorporated N from the shoots
occurred which reflected the N limitation. The
N limitation also affected carbon metabolism, in particular the flows
of C, incorporation in the shoot and
photosynthesis, which were decreased when compared with data from
recent experiments with pedospheric well
fed Ricinus. However, there was little difference in C
flows between nitrate and ammonium-sprayed plants with
respect to respiration, C partitioning and, most interestingly, in
relative stimulation of root growth. The loss of
C from dark respiration of the shoots was high on a f. wt basis
as well as in relative terms, owing to exclusive N
assimilation in the shoot. In general the plants invested untargeted
increases in root growth as a result of N
limitation irrespective of the imposed artificial treatment which
made the shoot the site of mineral N uptake.