Search

Skip to main content Accessibility help

Home
Search

Only show content I have access to (0)

Articles (1)

Over 3 years (1)

From year:

To year:

Apply

genetically modified soybean

Chinese Journal of Agricultural Biotechnology (1)

View selected items
Save to my bookmarks
Export citations
Download PDF (zip)
Save to Kindle
Save to Dropbox
Save to Google Drive
Save content to
To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to .

To save content items to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Please be advised that item(s) you selected are not available.
You are about to save
Your Kindle email address

Please provide your Kindle email.

@free.kindle.com @kindle.com (service fees apply)

By using this service, you agree that you will only keep content for personal use, and will not openly distribute them via Dropbox, Google Drive or other file sharing services

1 results

Detecting genetically modified Roundup Ready soybean ingredients in foodstuffs by nested and semi-nested PCR
Huang Kun-Lun, Luo Yun-Bo
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 1 / Issue 1 / April 2004

Published online by Cambridge University Press:

12 February 2007, pp. 31-36

Print publication:

April 2004
- Article
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation
DNA was extracted from kernels of genetically modified (GM) Roundup Ready soybean (Glycine max), made to a series of dilutions, and then used template for amplification by nested PCR and semi-nested PCR. The template comprising a 10−14 g/μl DNA solution could be amplified by both nested and semi-nested PCR. Various soybean foodstuffs and foods from a local supermarket were tested by nested and semi-nested PCR. The soybean housekeeping gene lectin could be amplified from 10 types of food (17 different brands), including soybean oil, sauce, lecithin, tofu, soybean milk and soy milk. The CaMV35S-CPT4 segment was amplified from eight types of food (14 brands), indicating that these foods were manufactured using soybean Roundup Ready ingredients. Therefore, nested and semi-nested PCR could serve as efficient methods in detecting GM soybean in foodstuffs and foods.